ABSTRACT
OBJECTIVE: The goal of this study was to determine the relationship between peak bone density and polymorphism of estrogen receptor gene in Korean premenopausal women. METHOD: We amplified Estrogen receptor by polymerase chain reaction and its digestion pattern of Pvu II and Xba I was examined from the 172 women who were in premenopause. The genetic subtype was classified by its digestion pattern and its combination genotype, e.g. PP, Pp, pp, XX, Xx, xx, PPXX, PPXx, PPxx, PpXx, Ppxx, xxpp. Bone mineral density of each genetic subtype were compared. RESULTS: The genotype distribution was as follows: PP; 16.3%, Pp; 45.9%, pp; 37.8%, XX; 1.2%, Xx; 25.0%, xx; 73.8%, PPXX; 1.1%, PPXx; 8.7%, PPxx; 6.4%, PpXx; 16.3%, Ppxx; 29.7%, ppxx; 37.8%. ER gene polymorphism was associated with bone mineral density at forearm bone mineral density (P<0.05) with the xx genotype having the less BMD than Xx genotype. CONCLUSION: Xba I estrogen receptor gene polymorphism is associated with bone mineral density in premenopausal women. Estrogen receptor gene polymorphism may have a modulatory role in bone metabolism in premenopausal women. And it can be used as predictor of forearm peak bone density.
Subject(s)
Female , Humans , Bone Density , Digestion , Estrogens , Forearm , Genotype , Metabolism , Polymerase Chain Reaction , PremenopauseABSTRACT
OBJECTIVE: To validate the efficacy of Patho_Net system for cervical cytology screening. METHODS: The results of imaging diagnosis by Patho_Net system were compared to those of 102 cervical smears diagnosed by conventional light microscopy. RESULTS: There was 52.9% concordant results. The image result for the slide were 41 (80.4%) WNL or atrophy, 19 (61.3%) inflammation or benign atypia, 1 (20%) LSIL, 2 (20%) HSIL and 1 (100%) invasive squamous cell carcinoma. CONCLUSION: Patho_Net system has potential for diagnostic value as a valid alternative to the traditional glass slide screening of Pap smear.
Subject(s)
Atrophy , Carcinoma, Squamous Cell , Diagnosis , Glass , Inflammation , Mass Screening , Microscopy , Papanicolaou Test , Vaginal SmearsABSTRACT
OBJECTIVE: To validate the efficacy of Patho_Net system for cervical cytology screening. METHODS: The results of imaging diagnosis by Patho_Net system were compared to those of 102 cervical smears diagnosed by conventional light microscopy. RESULTS: There was 52.9% concordant results. The image result for the slide were 41 (80.4%) WNL or atrophy, 19 (61.3%) inflammation or benign atypia, 1 (20%) LSIL, 2 (20%) HSIL and 1 (100%) invasive squamous cell carcinoma. CONCLUSION: Patho_Net system has potential for diagnostic value as a valid alternative to the traditional glass slide screening of Pap smear.
Subject(s)
Atrophy , Carcinoma, Squamous Cell , Diagnosis , Glass , Inflammation , Mass Screening , Microscopy , Papanicolaou Test , Vaginal SmearsABSTRACT
Embryonal rhabdomyosarcoma, called sarcoma botryoides, is the most common malignant tumor arising from in the infant vagina. This tumor is diagnosed before the age of 5 and highly malignant. We experienced a case of embryonal rhabdomyosarcoma arising from vaginal wall in 5-year-old girl. Authors presented this case with a brief review of literature.
Subject(s)
Child , Child, Preschool , Female , Humans , Infant , Rhabdomyosarcoma, Embryonal , Sarcoma , VaginaABSTRACT
OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.
Subject(s)
Female , Humans , Apoproteins , Cholesterol, HDL , Folic Acid , Genotype , Homocysteine , Methylenetetrahydrofolate Reductase (NADPH2) , Mutation, Missense , Plasma , Polymorphism, Genetic , Pre-Eclampsia , Pregnant Women , Prevalence , Risk Factors , Triglycerides , Vitamin B 12 , VitaminsABSTRACT
OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.